|
KMID : 0381120130350020185
|
|
Genes and Genomics
2013 Volume.35 No. 2 p.185 ~ p.195
|
|
|
Molecular cloning, expression profiles and subcellular localization of cyclin B in ovary of the mud crab, Scylla paramamosain
|
|
Li Wen-Xing
Huang Hui-Yang
Huang Jing-Ru
Yu Jin-Jin
Ma Jun
Ye Hai-Hui
|
|
|
Abstract
|
|
|
|
A full-length cDNA of cyclin B was isolated from ovary of the mud crab (Scylla paramamosain) in this study. This transcript encodes a polypeptide of 401 amino acids, which is highly homologous to cyclin B protein family. Reverse transcription-PCR (RT-PCR) showed that cyclin B mRNA was expressed at highest levels in ovary of the mud crab. During the ovarian maturation process, real-time RT-PCR revealed that the abundance of cyclin B mRNA increased from the second stage (early-developing stage) to the fourth stage (nearly-ripe stage) and reached the peak level at the fifth stage (ripe stage). This result indicates the identified cyclin B gene might be related to the cell proliferation in ovary, both mitotically and meiotically. Immunohistochemistry showed that cyclin B protein was localized in the cytoplasm of prophase oocytes at the second stage while enriched in the nuclei of pro-metaphase oocytes at the fourth stage. It suggests the tested cyclin B protein might play different roles in ovary at the two stages.
|
|
|
KEYWORD
|
|
|
Cyclin B, Scylla paramamosain, Ovary, cDNA cloning, Differential expression, Subcellular localization
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|